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Status |
Public on Jan 28, 2021 |
Title |
RMac transferred into clodronate-depleted recipients D31 |
Sample type |
RNA |
|
|
Source name |
Peritoneal cavity
|
Organism |
Mus musculus |
Characteristics |
donor treatment: Naive host treatment: Clodronate-depleted matrix: 2
|
Extracted molecule |
total RNA |
Extraction protocol |
For each of the indicated donor populations 5000 cells were sorted into 2µl of RLT (QIAGEN). Immediately after cells where: centrifuged at maximum speed 15 seconds, vortexed for 10 seconds and again centrifuged at maximum speed 15 seconds. Cells where stored at -80degrees C until analysis of the lysate using the nCounter Myeloid innate immunity panel.
|
Label |
n.a.
|
Label protocol |
n.a.
|
|
|
Hybridization protocol |
n.a.
|
Scan protocol |
n.a.
|
Description |
clodronate-depleted recipient
|
Data processing |
Data were imported into nSolver 4.0 software (Nanostring Technologies). Data were annotated by cell-type for analysis (RMac, RMacZ10, IMacZ10).Grouped data were then analysed using the advanced analysis nSolver package according to the manufacturers instructions. Importantly, donor populations transferred into mirroring (D1-D17) or clodronate-depleted (D20-D37) recipients were obtained on different days and were analysed separately.
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|
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Submission date |
Jan 18, 2021 |
Last update date |
Jan 28, 2021 |
Contact name |
Pieter Adriaan Louwe |
Organization name |
University of Edinburgh
|
Department |
Centre for Inflammation Research
|
Lab |
Jenking laboratory
|
Street address |
47 Little France Crescent
|
City |
Edinburgh |
State/province |
Scotland |
ZIP/Postal code |
EH15 1JA |
Country |
United Kingdom |
|
|
Platform ID |
GPL25266 |
Series (1) |
GSE165036 |
Nanostring analysis of macrophage subsets following zymosan-induced peritonitis |
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