|
| Status |
Public on Mar 04, 2021 |
| Title |
Control-2 |
| Sample type |
SRA |
| |
|
| Source name |
blood
|
| Organism |
Macaca fascicularis |
| Characteristics |
treatment: injected with PBS
tissue: blood
Sex: male
age: 3 years old
weight: about 2.5kg
|
| Growth protocol |
On day 4 p.i., blood was collected from nonpregnant experimental monkeys. Total RNA was extracted and purified. The purity, molar concentration and integrity of RNA were determined by Nanodrop, Qubit 2.0, and Aglient 2100, respectively, to ensure that the samples used for transcriptomic sequencing were qualified.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cell by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 4200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Reads filtering under criteria removing reads with 20% of the base quality lower than 13
HISAT2.1.0 mapping to reference genome
count counting
Genome_build: MacFas_5.0
Supplementary_files_format_and_content: TXT file contain counts for each sample
|
| |
|
| Submission date |
Jan 21, 2021 |
| Last update date |
Mar 05, 2021 |
| Contact name |
Liangquan Zhu |
| E-mail(s) |
1367391894@qq.com, sunshijing@dbn.cn
|
| Phone |
18510195460
|
| Organization name |
China Institute of Veterinary Drug Control
|
| Street address |
No. 8, Zhongguancun South Street, Haidian District
|
| City |
Beijing |
| ZIP/Postal code |
100081 |
| Country |
China |
| |
|
| Platform ID |
GPL23096 |
| Series (1) |
| GSE165283 |
Safety and Transcriptome Analysis of Brucella Live Attenuated Vaccine Strain S2 on Cynomolgus Monkeys |
|
| Relations |
| BioSample |
SAMN17487050 |
| SRA |
SRX9912257 |
