|
| Status |
Public on Dec 31, 2010 |
| Title |
TNNT2sp zebrafish replicate 3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Whole embryo TNNT2sp morphants
|
| Organism |
Danio rerio |
| Characteristics |
tissue: whole embryo
developmental stage: 96hpf
|
| Treatment protocol |
Embryos were injected with a control or TNNT2sp morpholino at the 1-4cell stage. They were then allowed to develop at 28 degrees Celsius for 96hrs.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Groups of 30 control or TNNT2sp morphant embryos were collected at 96hpf and RNA extracted with Trizol (Invitrogen). RNA was further processed with RNeasy purification columns (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
5 µg of total RNA from each sample was labeled using the Agilent Low RNA Input Kit (T7 promoter primer, Cy3 or Cy5 dyes)
|
| |
|
| Channel 2 |
| Source name |
Whole embryo control morphants
|
| Organism |
Danio rerio |
| Characteristics |
tissue: whole embryo
developmental stage: 96hpf
|
| Treatment protocol |
Embryos were injected with a control or TNNT2sp morpholino at the 1-4cell stage. They were then allowed to develop at 28 degrees Celsius for 96hrs.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Groups of 30 control or TNNT2sp morphant embryos were collected at 96hpf and RNA extracted with Trizol (Invitrogen). RNA was further processed with RNeasy purification columns (Qiagen)
|
| Label |
Cy5
|
| Label protocol |
5 µg of total RNA from each sample was labeled using the Agilent Low RNA Input Kit (T7 promoter primer, Cy3 or Cy5 dyes)
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Images were quantified using Agilent Feature Extraction Software
|
| Description |
Biological replicate 3 of 4
|
| Data processing |
Agilent Feature Extraction Software was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Feb 03, 2010 |
| Last update date |
Dec 31, 2010 |
| Contact name |
Jason Becker |
| Organization name |
Massachusetts General Hospital
|
| Department |
CVRC
|
| Lab |
MacRae Lab
|
| Street address |
13th St, Building 149
|
| City |
Charlestown |
| State/province |
MA |
| ZIP/Postal code |
02129 |
| Country |
USA |
| |
|
| Platform ID |
GPL6457 |
| Series (1) |
| GSE20179 |
Transcriptional profiling of embryonic zebrafish expressing a mutant TNNT2 |
|
