|
Status |
Public on Apr 26, 2021 |
Title |
B Cell - rep 2 |
Sample type |
SRA |
|
|
Source name |
B Cell
|
Organism |
Gallus gallus |
Characteristics |
strain: F1 crosses of Line 6 and Line 7 from the Avian Disease and Oncology Laboratory (ADOL) tissue: B Cell
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from tissues and immune cells was collected using Trizol/Chloroform. The total RNA from immune cells was DNase treated after extraction ERCC RNA Spike-In Controls included. Libraries were pooled and sequenced on HiseqX-PE150 by Novogene Bioinformatics Technology Co
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
HiSeq X Ten |
|
|
Description |
bcell.bw
|
Data processing |
TrimGalore (v0.4.1) was used to remove adapters STAR (v2.6.1c) was used for alignment deepTools bamCoverage (v3.5.0) was used to generate bigWig files Genome_build: GRCg6a (genome-build-accession NCBI:GCA_000002315.5) Supplementary_files_format_and_content: UCSC track format (bigWig)
|
|
|
Submission date |
Feb 05, 2021 |
Last update date |
Apr 26, 2021 |
Contact name |
David Hawkins |
E-mail(s) |
rdhawk@uw.edu
|
Organization name |
University of Washington
|
Department |
Division of Medical Genetics, Department of Genome Sciences
|
Street address |
1959 NE Pacific St, HSB K226
|
City |
Seattle |
State/province |
WA |
ZIP/Postal code |
98195 |
Country |
USA |
|
|
Platform ID |
GPL24517 |
Series (1) |
GSE166257 |
Chicken (Gallus gallus) Transcriptome of Intestinal, Immune, and Reproductive Cell and Tissue Types |
|
Relations |
BioSample |
SAMN17812695 |
SRA |
SRX10039804 |