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Sample GSM5067014 Query DataSets for GSM5067014
Status Public on Apr 26, 2021
Title B Cell - rep 2
Sample type SRA
 
Source name B Cell
Organism Gallus gallus
Characteristics strain: F1 crosses of Line 6 and Line 7 from the Avian Disease and Oncology Laboratory (ADOL)
tissue: B Cell
Extracted molecule total RNA
Extraction protocol Total RNA from tissues and immune cells was collected using Trizol/Chloroform. The total RNA from immune cells was DNase treated after extraction
ERCC RNA Spike-In Controls included. Libraries were pooled and sequenced on HiseqX-PE150 by Novogene Bioinformatics Technology Co
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Description bcell.bw
Data processing TrimGalore (v0.4.1) was used to remove adapters
STAR (v2.6.1c) was used for alignment
deepTools bamCoverage (v3.5.0) was used to generate bigWig files
Genome_build: GRCg6a (genome-build-accession NCBI:GCA_000002315.5)
Supplementary_files_format_and_content: UCSC track format (bigWig)
 
Submission date Feb 05, 2021
Last update date Apr 26, 2021
Contact name David Hawkins
E-mail(s) rdhawk@uw.edu
Organization name University of Washington
Department Division of Medical Genetics, Department of Genome Sciences
Street address 1959 NE Pacific St, HSB K226
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
 
Platform ID GPL24517
Series (1)
GSE166257 Chicken (Gallus gallus) Transcriptome of Intestinal, Immune, and Reproductive Cell and Tissue Types
Relations
BioSample SAMN17812695
SRA SRX10039804

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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