|
| Status |
Public on Feb 01, 2011 |
| Title |
mutant, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila 5-day-old adult male head, mutant sample
|
| Organism |
Drosophila melanogaster |
| Characteristics |
gender: male
developmental stage: 5-day-old adult
tissue: head
genotype: homozygous dme-miR-14 mutant
|
| Treatment protocol |
Head tissue was separated by snap freezing flies in liquid nitrogen and then vortexing.
|
| Growth protocol |
5-day-old male flies reared in similar growth and feeding conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was done.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
|
| |
|
| Hybridization protocol |
Using standard protocols, the cRNA was hybridized on Affymetrix Drosophila Genome 2.0 array. Washes were done using the Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from adult male mutant flies.
COHEDRO_03
|
| Data processing |
The data were analyzed using CARMAweb 1.4, a comprehensive R- and Bioconductor-based web service for microarray data analysis, using settings specific for Affymetrix using RMA method.
|
| |
|
| Submission date |
Feb 04, 2010 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jishy Varghese |
| E-mail(s) |
jishy@tll.org.sg
|
| Phone |
0065 91894341
|
| Fax |
0065 91894341
|
| Organization name |
TLL, Singapore
|
| Street address |
1 Research Link
|
| City |
Singapore |
| ZIP/Postal code |
117604 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE20202 |
Expression data from adult male Drosophila heads |
|
| Relations |
| Reanalyzed by |
GSE119084 |
