|
| Status |
Public on Apr 20, 2010 |
| Title |
1-6_AtGA120.2_Rep2_ATH1 |
| Sample type |
RNA |
| |
|
| Source name |
GA2oxOE1I, 120 min
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
developmental stage: 7 days
tissue: root tips
genetic background: Col-0
genetic variation: gene knock out
treatment: GA
treatment time: 120 min.
|
| Growth protocol |
Roots were grown on 100mm x 100mm square plates stood at an angle of 70ยบ from the horizontal. Plants were grown on 0.7% Gelrite (Duchefa), 0.05% MES (Sigma), 1x Murashige and Skoog (Duchefa), 1% Sucrose (Duchefa) at pH 5.8. Plants were grown in continuous light and harvested when they were 7 days old.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy kit protocol
|
| Label |
biotin
|
| Label protocol |
Labelled using protocol described in manual "Affymetrix GeneChip Expression Analysis Technical Manual", One-Cycle target labelling
|
| |
|
| Hybridization protocol |
Biotin Labelled cRNA using "Affymetrix GeneChip Hybridization, Wash and Stain Kit", according to guidelines set out by Affymetrix, Santa Clara, CA
|
| Scan protocol |
Scanned according to guidelines set out by Affymetrix, Santa Clara, CA.
|
| Description |
Seeds were imbibed in water for 3 days at 4C. Seeds were sterilsed for 3 minutes in 70% ethanol, 3 minutes in 10% bleach/0.1% Tween-20 and rinsed three times in water. Growth Room Phytagar 90% 22
|
| Data processing |
Raw data from the microarrays was normalized at probe-level using MAS5 algorithm. The detection calls (present, marginal, absent) for each probe set was obtained using the GCOS system
|
| |
|
| Submission date |
Feb 08, 2010 |
| Last update date |
Apr 19, 2010 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
affy@arabidopsis.info
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE20223 |
Gibberellin (GA) Treatment of Arabidopsis Root Tips |
|
