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Sample GSM5070539 Query DataSets for GSM5070539
Status Public on Mar 31, 2021
Title Brain_Young_LL_P22547
Sample type SRA
 
Source name Brain
Organism Peromyscus leucopus
Characteristics stock: LL
tissue: Brain
age of animal at termination: 7 months
Treatment protocol Brain tissues of 7-months-old and 2- to 3-years-old outbred deer mice P. maniculatus and P. leucopus were collected at sacrifice.
Growth protocol Deer mice, P. maniculatus were obtained from the Peromyscus Genetic Stock Center (PGSC), University of South Carolina (USC), Columbia, SC (RRID:SCR_002769). P. maniculatus bairdii (BW Stock), was closed colony bred in captivity since 1948 and descended from 40 ancestors wild-caught near Ann Arbor, Michigan. P. leucopus (LL Stock) was derived from 38 wild ancestors captured between 1982 and 1985.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from brain using the RNeasy Mini Kit (Qiagen).
Sequencing libraries were generated using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB#E7490/NEB#E7760).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
 
Description S05
RNASeqCounts_LL.txt
Data processing Indexed sequences were checked by FastQC | version: 0.11.5.
Trimmed adapter by TrimGalore-0.4.5.
Reads were mapped to the P. maniculatus and P. leucopus reference genome using STAR | version 2.7.2b with default parameters (generation of sam files).
Convert .sam files to .bam files using samtools | version: 1.5 with parameters -bh -F 3840 -q 255.
Read counts over annotated genes Peromyscus_maniculatus_bairdii.HU_Pman_2.1.101.gtf and GCF_004664715.1_Pero_0.1_genomic.gtf were obtained using featureCount (subreads | version: 1.5.3) with parameters -p -T 10.
Genome_build: Peromyscus_maniculatus_bairdii.HU_Pman_2.1 and GCF_004664715.1_Pero_0.1_genomic
Supplementary_files_format_and_content: RNASeqCounts_*.txt: Tab-delimited text files include gene counts for each sample.
 
Submission date Feb 08, 2021
Last update date Mar 31, 2021
Contact name HAO JI
E-mail(s) ji5@email.sc.edu
Phone 8037774689
Organization name University of South Carolina
Department College of Pharmacy
Street address 715 SUMTER ST CLS RM 109
City COLUMBIA
State/province SC
ZIP/Postal code 29208
Country USA
 
Platform ID GPL29708
Series (1)
GSE166394 Whole Transcriptome RNA-seq in Brain Tissue of Young and Old Peromyscus maniculatus and P. leucopus
Relations
BioSample SAMN17836388
SRA SRX10052164

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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