|
| Status |
Public on Feb 20, 2021 |
| Title |
MycER-BclXL-Fzd9 wt mouse 4 |
| Sample type |
RNA |
| |
|
| Source name |
Insulinoma from MycER-BclXL-Fzd9 wt mouse
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Fzd9 wt
tumor type: Insulinoma
|
| Treatment protocol |
Mice were treated with Tamoxifen i.p. for three days
|
| Growth protocol |
MycER;BclXl Fzd9 wt and Fzd9-/- C57Bl/6 mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
50-100 mg of tumor tissue was homogenized with a pestle in TRIzol. Total RNA were extracted using TRIzol following manufacturer's procedures. Total RNA was quantified using a NanoDrop Spectrophotometer ND-1000 (NanoDrop Technologies, Inc.) and its integrity was assessed using a 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
Biotin
|
| Label protocol |
Labeling of samples was carried out according to the manufacturer’s standard protocol
|
| |
|
| Hybridization protocol |
Total RNA was linearly amplified using WT Plus (Affymetrix). The resulting ssDNA was used to hybridize Affimetrix microarrays. Hybridization data was acquired using the Affimetrix GeneChip/GeneTitan platforms. Transcriptomes were determined on a genome wide Mouse Gene 2.1 Array (Affimetrix).
|
| Scan protocol |
Arrays were scanned on a Affymetrix GeneTitan instrument (ThermoFisher Scientific-Affymetrix) according to the manufacturer’s protocol.
|
| Data processing |
Affymetrix CEL files were normalized by RMA preprocessing methodology and analyzed using Partek Genomic Suite followed by GSEA 3.0 software from Broad Institute.
|
| |
|
| Submission date |
Feb 19, 2021 |
| Last update date |
Feb 20, 2021 |
| Contact name |
Mariano F Zacarias-Fluck |
| E-mail(s) |
mzacarias@vhio.net
|
| Organization name |
Vall d'Hebron Institute of Oncology (VHIO)
|
| Department |
Preclinical and Translational Research Program
|
| Lab |
Mouse Models of Cancer Therapy
|
| Street address |
Natzaret, 115-117
|
| City |
Barcelona |
| ZIP/Postal code |
08035 |
| Country |
Spain |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE167073 |
Effect of Fzd9 knock-out on MycER-BclXl insulinoma progression |
|
