|
| Status |
Public on Oct 13, 2010 |
| Title |
Glycine soja, young leaves, 50 mM NaHCO3, 30 minutes, replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
leaf after 0.5h stress
|
| Organism |
Glycine soja |
| Characteristics |
line: G07256
tissue: leaf
age: 21 days after germination
treatment: NaHCO3
time: 0.5h
|
| Treatment protocol |
21-day-old seedlings were transferred into the growth boxes containing 1/4 strength Hoagland’s solution with 50mmol/L NaHCO3 (pH 8.5).
|
| Growth protocol |
Glycine soja seeds were grown in a culture room with 60 % relative humidity, 24℃ and a light regime of 12h light/12h dark. Germinated seedlings were grown in the growth boxes containing 1/4 strength Hoagland's solution.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from frozen leaves with TRIzol (Invitrogen, Carlsbad, CA) according to the instructions from the manufacturer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Soybean Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from Glycine soja leaves of 21 days old after 0.5h NaHCO3 stress.
0.5h_leaf_r1
|
| Data processing |
The computation of expression values was conducted using dChip software. Sample wise normalization to the median probe cell intensity (CEL) of all 14 arrays. Model-based gene expression was obtained from normalized CEL intensities based on a Perfect Match-only model.
|
| |
|
| Submission date |
Feb 13, 2010 |
| Last update date |
Oct 13, 2010 |
| Contact name |
Yanming Zhu |
| E-mail(s) |
ymzhu2001@neau.edu.cn
|
| Phone |
86-451-55190734
|
| Organization name |
Northeast Agriculture University
|
| Department |
Life sciences
|
| Lab |
Plant Bioengineering laboratory
|
| Street address |
Wood Street 59
|
| City |
Harbin |
| State/province |
Heilongjiang |
| ZIP/Postal code |
150030 |
| Country |
China |
| |
|
| Platform ID |
GPL4592 |
| Series (1) |
| GSE20323 |
Alkaline-stress response in Glycine soja leaf identifies specific transcription factors and ABA-mediated signaling factors |
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