|
| Status |
Public on Mar 01, 2021 |
| Title |
OCExoInhib SeraExo#3 |
| Sample type |
RNA |
| |
|
| Source name |
Serum exosomes
|
| Organism |
Mus musculus |
| Characteristics |
sample type: Exosomes
|
| Treatment protocol |
The exosomes were isolated from serum of OCExoInhib-treated mice or Vehicle-treated mice.
|
| Growth protocol |
The mice with OCExoInhib treatment and control mice with vehicle treatment at 2w after ACLT.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Exosomal RNA was extracted with the SeraMir Exosome RNA Column Purification Kit (System Biosciences) according to the manufacturer's instructions.
|
| Label |
SYBR Green
|
| Label protocol |
Reverse transcription was performed using the Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit (System Biosciences) according to the manufacturer's instructions. Real-time qPCR was performed (Applied Biosystems ViiA7) using standard run conditions (40 cycles at 95oC for 15 seconds and 60oC for 60 seconds.)
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
OCExoInhib treatment
SAMPLE 6
|
| Data processing |
The normalization and all the data analysis was performed using the manufacturer's qPCR analysis templates https://systembio.com/wp-content/uploads/Mouse_miRNome_v14_2.xls
For the normalization it uses the Spike-in control RNA.
Target miRNA signals were normalized to the average Spike-in control RNA signals; 2^-deltaCt, where deltaCt = (Ct_Target - Ct_control average)]
All deltadeltaCt based fold-change were calculated from the uploaded raw threshold cycle data automatically by the templates.
Fold Change worksheet reports test/control (i.e., OCExoInhib/Vehicle) ratios.
|
| |
|
| Submission date |
Feb 21, 2021 |
| Last update date |
Mar 01, 2021 |
| Contact name |
Jin Liu |
| E-mail(s) |
drlausums@gmail.com
|
| Organization name |
Hong Kong Baptist University
|
| Department |
TMBJ
|
| Street address |
7 Baptist University Road, Kowloon Tong
|
| City |
Hong Kong SAR |
| ZIP/Postal code |
999077 |
| Country |
China |
| |
|
| Platform ID |
GPL29749 |
| Series (1) |
| GSE167160 |
Real-time microRNA quantitative PCR array of serum exosomes I |
|
