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Sample GSM5098823 Query DataSets for GSM5098823
Status Public on Jun 06, 2023
Title PE-cortex-1
Sample type SRA
 
Source name Cortex
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: Cortex offspring from preeclampsia mother mice
devolopmental stage: fetus at E17.5 stage
Extracted molecule total RNA
Extraction protocol E17.5 cortexes were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Data processing Reads filtering under criteria removing reads with 20% of the base quality lower than 13
HISAT2.1.0 mapping to reference genome
count counting
Genome_build: GRCm38
Supplementary_files_format_and_content: TXT file contain counts for each sample
 
Submission date Feb 22, 2021
Last update date Jun 06, 2023
Contact name Wen-Long Zhao
E-mail(s) zbyzwl1919@sina.com
Organization name The International Peace Maternity and Child Health Hospital
Street address Guangyuan road 145#
City Shanghai
ZIP/Postal code 200030
Country China
 
Platform ID GPL21273
Series (1)
GSE167193 Next Generation Sequencing Facilitates Quantitative Analysis of transcriptome profiling (RNA-seq) of E17.5 cortex from offspring derived from control and preeclampsia mothers
Relations
BioSample SAMN18024796
SRA SRX10148227

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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