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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 01, 2022 |
| Title |
Day0-4 (miRNA-seq) |
| Sample type |
SRA |
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| Source name |
whole body
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| Organism |
Aedes aegypti |
| Characteristics |
strain: Rockefeller
tissue: whole body
protocol: blood feeding
blood from: human donor 4
time: 1 hr
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| Treatment protocol |
7 days old mosquitos were following a blood meal, then cultured after 1hr or 6 days
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| Growth protocol |
Aedes Aegypti was reared at 28℃ and 80% humidity.
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| Extracted molecule |
total RNA |
| Extraction protocol |
The total mosquito RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
small RNA-Seq
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| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
BGISEQ-500 |
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| Data processing |
Remove low quality tags
Remove tags with 5' primer contaminants
Remove tags without 3' primer
Remove tags without insertion
Remove tags with poly A
Remove tags shorter than 18nt
Genome_build: miRBase 22.1 and Rfam 14.4
Supplementary_files_format_and_content: Excel files include RPKM values, log2 ratio, P values, FDR, KEGG Orthology, and GO analysis for each Sample ...
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| Submission date |
Feb 23, 2021 |
| Last update date |
May 01, 2022 |
| Contact name |
Yibin Zhu |
| E-mail(s) |
zhuyibin1989@126.com
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| Phone |
18515653980
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| Organization name |
Tsinghua University
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| Street address |
Tsinghua, Beijing, China
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| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platform ID |
GPL29762 |
| Series (2) |
| GSE167346 |
A human blood-derived microRNA facilitates flavivirus infection in fed mosquitoes [miRNA-seq] |
| GSE167348 |
A human blood-derived microRNA facilitates flavivirus infection in fed mosquitoes |
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| Relations |
| BioSample |
SAMN18038667 |
| SRA |
SRX10157100 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM5102591_Day0-4.expr.txt.gz |
17.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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