|
| Status |
Public on Feb 28, 2021 |
| Title |
IN54-Cas9-gRNA-unedited-2 |
| Sample type |
SRA |
| |
|
| Source name |
Muscle-derived stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Muscle
passages: 4
treatment: IN54-Cas9-gRNA untreated
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
The cells were trypsinized and collected, and genomic DNA was extracted using the TIANamp Genomic DNA Kit (TIANGEN)
Amplification primers containing Illumina forward and reverse adapters were used for a first round of PCR (PCR 1) amplifying the genomic region of interest. Unique Illumina barcoding primer pairs were added to each sample in a secondary PCR reaction (PCR 2).
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Quantification_of_editing_reads_counts_and_frequency.xls
|
| Data processing |
Library strategy: Amplicon-seq
High-throughput sequencing was performed on NOVA SEQ system (Illumina).
Trimmed reads were obtained by removing adapter sequences, and reads with low complexity or of low quality were removed.
The types and proportions of SNVS are analyzed using customized shell scripts
Genome_build: hg38
|
| |
|
| Submission date |
Feb 25, 2021 |
| Last update date |
Feb 28, 2021 |
| Contact name |
Shixue Gou |
| Organization name |
GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH,CHINESE ACADEMY OF SCIENCES
|
| Street address |
No. 190, Kaiyuan Avenue, Huangpu District, Guangzhou
|
| City |
Guangzhou |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE167572 |
In vivo genome editing restores dystrophin expression in Duchenne muscular dystrophy patient muscle fibers [Amplicon-seq] |
| GSE168007 |
In vivo genome editing restores dystrophin expression in Duchenne muscular dystrophy patient muscle fibers |
|
| Relations |
| BioSample |
SAMN18059935 |
| SRA |
SRX10171554 |
