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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 10, 2010 |
| Title |
mouse embyronic stem cell#6 |
| Sample type |
SRA |
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| Source name |
ES cell
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| Organism |
Mus musculus |
| Characteristics |
gender: female
sample type: ES cell
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
whole cell lysis, Whole transcriptome Amplification by RT-PCR from a single cell, mRNA with poly(A) tail
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
AB SOLiD System |
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| Description |
Data was processed with base quality values
AB SOLiD system V3.0
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| Data processing |
srf conversion generated by SOLiD srt tool: http://solidsoftwaretools.com/gf/project/srf/
raw data alignet to Mouse genome (Jul. 2007 assembly of the mouse genome: mm9, NCBI Build 37) using SOLiD whole transcriptome pipeline: http://solidsoftwaretools.com/gf/project/transcriptome/
algorithm description: Tang F, Barbacioru C, Wang Y, Nordman E, Lee C, Xu N, Wang X, Bodeau J, Tuch BB, Siddiqui A, Lao K, Surani MA., mRNA-Seq whole-transcriptome analysis of a single cell, Nat Methods. 2009 May;6(5):377-82.
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| Submission date |
Feb 20, 2010 |
| Last update date |
Jun 11, 2013 |
| Contact name |
Catalin Barbacioru |
| E-mail(s) |
catalin@appliedbiosystems.com
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| Organization name |
Applied Biosystems
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| Street address |
850 Lincoln Centre Dr
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| City |
Foster City |
| State/province |
CA |
| ZIP/Postal code |
94404 |
| Country |
USA |
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| Platform ID |
GPL10010 |
| Series (1) |
| GSE20187 |
Tracing the derivation of embryonic stem cells from the inner cell mass by single cell RNA-seq analysis |
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| Relations |
| BioSample |
SAMN02196265 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM512602_ESC_A6.max.srf.tar.gz |
59.6 Mb |
(ftp)(http) |
TAR |
| Processed data provided as supplementary file |
| Raw data are available on Series record |
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