|
Status |
Public on Mar 02, 2024 |
Title |
Serum-LungCancer-rep29-Phase II |
Sample type |
protein |
|
|
Channel 1 |
Source name |
Serum,LungCancer,Phase II
|
Organism |
Homo sapiens |
Characteristics |
gender: female sampletype: Serum cancertype: LungCancer smoke: no family_history: no tuberculosis_history: no surgery: yes designphase: Phase II molecule: Serum autoantibody proteins IgG
|
Extracted molecule |
protein |
Extraction protocol |
standard protocol
|
Label |
Cy3
|
Label protocol |
After washing with PBST, the microarray was incubated with 1:1000 diluted Fluor conjugated goat anti-human IgG (532nm) and donkey anti-human IgM (635nm) (Jackson ImmunoResearch, West Grove, PA) at RT for 1h in the dark. After washing with PBST, the microarray was rinsed with dd H2O and dried
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|
|
Channel 2 |
Source name |
Serum,LungCancer,Phase II
|
Organism |
Homo sapiens |
Characteristics |
molecule: Serum autoantibody proteins IgM
|
Extracted molecule |
protein |
Extraction protocol |
standard protocol
|
Label |
Cy5
|
Label protocol |
After washing with PBST, the microarray was incubated with 1:1000 diluted Fluor conjugated goat anti-human IgG (532nm) and donkey anti-human IgM (635nm) (Jackson ImmunoResearch, West Grove, PA) at RT for 1h in the dark. After washing with PBST, the microarray was rinsed with dd H2O and dried
|
|
|
|
Hybridization protocol |
The microarray was taken out from − 80°C and then incu-bated in blocking buffer (3% BSA in PBS) at RT for 3h.Then a serum sample diluted at 1:200 in binding buffer (1%BSA in PBST) was added to the microarray and incubatedat 4°C overnight
|
Scan protocol |
The micro-array was scanned with the LuxscanTM 10K-A (CapitalBio Corporation, Beijing, China).
|
Description |
Phase II of the experimental design
|
Data processing |
Data analysis was carried out using The GenePix Pro 6.0 (Axon Instruments, Foster City, CA). The signal for each spot (SNR) was defined as the ratio of the fore-ground to the background median intensity and normalized using the median value.
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|
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Submission date |
Mar 03, 2021 |
Last update date |
Mar 02, 2024 |
Contact name |
He Yan Bin |
E-mail(s) |
heyanbin@bcbio.cn
|
Phone |
18565610792
|
Organization name |
BCBIO
|
Street address |
Sino-European Center A, 2 Lingnan Avenue South, Shunde, Foshan, Guangdong, China
|
City |
Foshan |
State/province |
GUANGDONG |
ZIP/Postal code |
528315 |
Country |
China |
|
|
Platform ID |
GPL29810 |
Series (1) |
GSE168198 |
Human serum: Control vs. Lung Cancer |
|