 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 01, 2024 |
| Title |
W2: BL21(DE3) Star_mfLon-rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Ecoli cells
|
| Organism |
Escherichia coli |
| Characteristics |
strain: BL21(DE3)
abbreviations in data: W2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Bcl2fastq (v2.17.1.14) software for base calling
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to E.coli BL21(DE3) whole genome using Bowtie v2.2.6.
Fragments Per Kilo bases per Million reads (FPKM) were calculated using a protocol from Mortazavi et al., Nature methods, 2008
Genome_build: E.coli BL21(DE3) （GenBank: CP001509.3）
Supplementary_files_format_and_content: Matrix table with raw gene counts and FPKM values for every gene and every sample
|
| |
|
| Submission date |
Mar 04, 2021 |
| Last update date |
Mar 01, 2024 |
| Contact name |
Hao Qi |
| Organization name |
Tianjin University
|
| Street address |
No. 135 Yaguan Road, Haihe Educaton Park
|
| City |
Tianjin |
| ZIP/Postal code |
300350 |
| Country |
China |
| |
|
| Platform ID |
GPL25368 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN18138974 |
| SRA |
SRX10237493 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
