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| Status |
Public on Mar 01, 2024 |
| Title |
C1: SfB-ABCFPfA_mfLon-rep1 |
| Sample type |
SRA |
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| Source name |
Ecoli cells
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| Organism |
Escherichia coli |
| Characteristics |
strain: SfB-ABCFPfA
abbreviations in data: C1
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
Bcl2fastq (v2.17.1.14) software for base calling
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to E.coli BL21(DE3) whole genome using Bowtie v2.2.6.
Fragments Per Kilo bases per Million reads (FPKM) were calculated using a protocol from Mortazavi et al., Nature methods, 2008
Genome_build: E.coli BL21(DE3) （GenBank: CP001509.3）
Supplementary_files_format_and_content: Matrix table with raw gene counts and FPKM values for every gene and every sample
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| Submission date |
Mar 04, 2021 |
| Last update date |
Mar 01, 2024 |
| Contact name |
Hao Qi |
| Organization name |
Tianjin University
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| Street address |
No. 135 Yaguan Road, Haihe Educaton Park
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| City |
Tianjin |
| ZIP/Postal code |
300350 |
| Country |
China |
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| Platform ID |
GPL25368 |
| Series (1) |
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| Relations |
| BioSample |
SAMN18138979 |
| SRA |
SRX10237498 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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