|
| Status |
Public on Feb 01, 2011 |
| Title |
WT-HCG2 |
| Sample type |
RNA |
| |
|
| Source name |
Granulosa Cell, 44-46hr PMSG, 6hr hCG
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J;129S5/SvEvBrd
genome/variation: wild type
cell type: granulosa cell
treatment group: 44-46hr PMSG, 6hr hCG
|
| Treatment protocol |
Granulosa cells collected by puncture of antral follicles; cells processed immediately, no further treatment
|
| Growth protocol |
21-day mice were injected with 5IU PMSG for 44-46 hours or 44-46 hours PMSG followed by 6 hr 5IU hCG
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted using Qiagen RNeasy Kit
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocols at Baylor Microarray Core, BCM
|
| |
|
| Hybridization protocol |
Affymetrix protocols at Baylor Microarray Core, BCM
|
| Scan protocol |
Affymetrix protocols at Baylor Microarray Core, BCM
|
| Description |
hnl_MM_2523_MA2_9750.cel
|
| Data processing |
GCRMA (Genespring 10.0.2, default parameters, baseline transformed to median of all samples)
|
| |
|
| Submission date |
Feb 22, 2010 |
| Last update date |
Feb 01, 2011 |
| Contact name |
Martin Matzuk |
| Organization name |
Baylor College of Medicine
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE20466 |
Comparison of granulosa cells from WT and Inha KO mice following gonadotropin stimulation |
|
