|
| Status |
Public on Feb 24, 2010 |
| Title |
WT_WD_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse islets
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: C57Bl/6Hsd
age: 10 months
tissue: islets
genotype/variation: WT
growth protocol: Western diet
|
| Treatment protocol |
To isolate the islets from pancreas, pancreas were perfused with collagenase solution.
|
| Growth protocol |
Mouse were fed chow and a western diet for 6 months. The diet regimen started when they were at 4 month old
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed using a 1% agarose gel.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina Totalprep kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1 of WTWD
|
| Data processing |
The data were normalised using quantile normalisation using Illumina's BeadStudio software
|
| |
|
| Submission date |
Feb 24, 2010 |
| Last update date |
Feb 24, 2010 |
| Contact name |
Yoonkwang Lee |
| E-mail(s) |
ylee3@neomed.edu
|
| Organization name |
Northeast Ohio Medical University
|
| Department |
Integrative Medical Sciences
|
| Street address |
4209 State Route 44
|
| City |
Rootstown |
| ZIP/Postal code |
44272 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE20518 |
mRNA expression in islets isolated from wild type and SHPKO mice fed chow or western diet |
|
