|
| Status |
Public on Oct 06, 2021 |
| Title |
Rod/Pineal Replicate 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
rod1
|
| Organism |
Danio rerio |
| Characteristics |
developmental stage: Adult
strain: Ekkwill
transgenic line: Tg(rho:egfp)ja2Tg
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the sorted cells using RNeasy Extraction kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Cy3- or Cy5- labeled cRNA probe was synthesized from 150 ng of total RNA using the Quick-Amp Labeling Kit (Agilent Technologies).
|
| |
|
| Channel 2 |
| Source name |
pineal2
|
| Organism |
Danio rerio |
| Characteristics |
developmental stage: Adult
strain: Ekkwill
transgenic line: Tg(exorh:EGFP)ja1Tg
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the sorted cells using RNeasy Extraction kit (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
Cy3- or Cy5- labeled cRNA probe was synthesized from 150 ng of total RNA using the Quick-Amp Labeling Kit (Agilent Technologies).
|
| |
|
| |
| Hybridization protocol |
Equal amounts of Cy3 and Cy5-labeled cRNA (825 ng) from two different samples were hybridized to zebrafish microarrays for 17 h at 60 °C.
|
| Scan protocol |
The hybridized arrays were then washed and scanned using an Agilent microarray scanner (G2505 C; Agilent Technologies)
|
| Data processing |
Data were extracted from the scanned image using Feature Extraction version 10.5.1.1 (Agilent Technologies).
|
| |
|
| Submission date |
Mar 11, 2021 |
| Last update date |
Oct 06, 2021 |
| Contact name |
Daisuke Kojima |
| E-mail(s) |
sdkojima@mail.ecc.u-tokyo.ac.jp
|
| Phone |
+81-3-5841-4382
|
| Organization name |
The University of Tokyo
|
| Department |
Department of Biological Sciences, School of Science
|
| Lab |
Laboratory of Biochemistry and Neuroscience
|
| Street address |
7-3-1 Hongo
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113*0033 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6457 |
| Series (1) |
| GSE168749 |
Transcriptional profiles of cone cells, rod cells, and pineal photoreceptor cells in zebrafish |
|
