|
| Status |
Public on Mar 17, 2021 |
| Title |
Monkey sperm - Maltreated 1 |
| Sample type |
SRA |
| |
|
| Source name |
sperm cells
|
| Organism |
Macaca mulatta |
| Characteristics |
age: 5.67 yr
group: Maltreated
|
| Treatment protocol |
PureSperm 40/80 density gradient
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol extraction protocol
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Data processing |
alignment (STAR)
counts stranded reads aligned to MacaM genome (featureCounts)
determine gene coverage by reads (bedtools)
Genome_build: MacaM
Supplementary_files_format_and_content: comma-separated text file, read counts, gene coverage
|
| |
|
| Submission date |
Mar 16, 2021 |
| Last update date |
Mar 17, 2021 |
| Contact name |
Dias Brian |
| Organization name |
Children's Hospital Los Angeles
|
| Street address |
4640 West Sunset Boulevard
|
| City |
Los Angeles |
| ZIP/Postal code |
90027 |
| Country |
USA |
| |
|
| Platform ID |
GPL23804 |
| Series (1) |
| GSE168978 |
Profiling non-human primate germline RNA to understand the legacy of early life stress |
|
| Relations |
| BioSample |
SAMN18318646 |
| SRA |
SRX10354425 |
