|
| Status |
Public on Mar 01, 2011 |
| Title |
Female rats vs male rats rep3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Liver from female Sprague-Dawley rat
|
| Organism |
Rattus norvegicus |
| Characteristics |
sex: Female
age: 7-weeks old
tissue: Liver
strain: Sprague-Dawley
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from frozen livers using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
5µg of total RNA was used for a first strand cDNA synthesis of each sample, labelled with either Cy3- or Cy5-dCTP. The microarray experiment was performed using the Pronto! Plus systems according to the manufacturer´s instructions (Corning and Promega).
|
| |
|
| Channel 2 |
| Source name |
Liver from male Sprague-Dawley rat
|
| Organism |
Rattus norvegicus |
| Characteristics |
sex: Male
age: 7-weeks old
tissue: Liver
strain: Sprague-Dawley
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from frozen livers using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
5µg of total RNA was used for a first strand cDNA synthesis of each sample, labelled with either Cy3- or Cy5-dCTP. The microarray experiment was performed using the Pronto! Plus systems according to the manufacturer´s instructions (Corning and Promega).
|
| |
|
| |
| Hybridization protocol |
Cy3- and Cy5 labelled samples were purifyed and mixed before addition of a hybridization buffer and added to respectively array for 18h of hybridization. After hybridization, the slides were washed multiple times, all according to the Pronto! Plus systems and the manufacturer´s instructions (Corning and Promega).
|
| Scan protocol |
Scanning was performed using a Genepix 4200A scanner (Axon).
Images were quantified using Genepix 6.0 software (Axon).
|
| Description |
Biological replicate 3 of 4.
|
| Data processing |
Background was subtracted using the in-house Easy Filter software. The data was normalized by the LOWESS method using the R language. The normalized data was finally statistically analyzed using SAM.
|
| |
|
| Submission date |
Mar 02, 2010 |
| Last update date |
Mar 01, 2011 |
| Contact name |
Carolina Gustavsson |
| Organization name |
Karolinska institutet
|
| Department |
Molecular medicine and surgery
|
| Lab |
Molecular endocrinology
|
| Street address |
CMM L8:01
|
| City |
Stockholm |
| ZIP/Postal code |
17176 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6528 |
| Series (1) |
| GSE20601 |
Sex-different hepatic glycogen content, glucose and amino acid output in rats. |
|
