|
| Status |
Public on Apr 22, 2021 |
| Title |
Gastruloid 0h sample 1 |
| Sample type |
SRA |
| |
|
| Source name |
Embryonic stem cell
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: WA01 (H1)
cell type: Embryonic stem cell
treatment: 0h (immediately before) BMP4 treatment
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
10x Genomcis Chromium single cell preparation
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Cells were encapsulted and library prepared using the 10X Chromium instrument with the Single Cell 3' Reagent Kits (v2 chemistry). BAM files and Gene-barcode matrices were generated using 10X Genomics Cellranger version 2
|
| Data processing |
Generation of fastq files via https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest
Alignment to hg19 genome build via https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest
Genome_build: hg19
Supplementary_files_format_and_content: counts.csv, metadata.csv
|
| |
|
| Submission date |
Mar 17, 2021 |
| Last update date |
Apr 23, 2021 |
| Contact name |
Kyaw Thu Minn |
| E-mail(s) |
ktm.minn@gmail.com
|
| Organization name |
WUSTL
|
| Street address |
1 Brookings Drive
|
| City |
St Louis |
| ZIP/Postal code |
63130 |
| Country |
USA |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE169074 |
Gene expression dynamics underlying cell fate emergence in 2D micropatterned human embryonic stem cell gastruloids |
|
| Relations |
| BioSample |
SAMN18341565 |
| SRA |
SRX10372575 |
