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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 15, 2021 |
| Title |
SC-siNC |
| Sample type |
SRA |
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| Source name |
mESC
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| Organism |
Mus musculus |
| Characteristics |
cell line: TC1
cell type: mESC cultured in serum/lif medium
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| Extracted molecule |
total RNA |
| Extraction protocol |
DNA and RNA extracted according to the manufacturer’s instructions.
Libraries of WGBS-seq, single-cell seq and ribo-seq constructed according to the manufacturer's instructions.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
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| Data processing |
For single-cell RNA seq, cellranger was used for the pre-processing and Seurat was used for the downstream analysis. Bismark was used for WGBS analysis. The company, Lc-bio Technologies, constructed the Ribo-seq libraries and performed the preliminary bioinformatics.
Genome_build: mm10
Supplementary_files_format_and_content: count, bismark.coverage
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| Submission date |
Mar 22, 2021 |
| Last update date |
May 16, 2021 |
| Contact name |
Min Yang |
| E-mail(s) |
yangmean@pku.edu.cn
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| Phone |
15210364662
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| Organization name |
Peking University
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| Street address |
No. 5, yiheyuan road, haidian district,
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platform ID |
GPL21273 |
| Series (1) |
| GSE168728 |
Mouse totipotent stem cells captured and maintained through spliceosomal repression |
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| Relations |
| BioSample |
SAMN18401790 |
| SRA |
SRX10405414 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM5195022_SC-siNC-count.csv.gz |
20.1 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Processed data provided as supplementary file |
| Raw data are available in SRA |
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