|
| Status |
Public on Mar 10, 2010 |
| Title |
MCF-7 P-62 |
| Sample type |
RNA |
| |
|
| Source name |
Epithelial cells derived from metastatic adenocarcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
age: 69 years
race: Caucasian
cell line: MCF-7
|
| Treatment protocol |
Stripped cells were grown over 96 hours (4 successive days) in phenol-red free IMEM media supplemented with 5% Charcoal-Stripped Calf Serum. Every 24 hours the media was removed and replaced with fresh phenol-red free IMEM media supplemented with 5% Charcoal-Stripped Calf Serum (3 times total).
|
| Growth protocol |
Cells were grown in complete IMEM media supplemented with 5% Fetal Bovine Serum. Both Control and Treated cells were kept in a 37C incubator with 5% C02 and regulated humidity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Standard one-cycle Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Protocol for Affymetrix GeneChip Scanner 3000
|
| Description |
n/a
|
| Data processing |
PLIER
|
| |
|
| Submission date |
Mar 09, 2010 |
| Last update date |
Mar 09, 2010 |
| Contact name |
Li Lily Chen |
| E-mail(s) |
lchen06@vt.edu
|
| Phone |
703-387-6060
|
| Fax |
703-528-5543
|
| Organization name |
Virginia Tech
|
| Department |
The Bradley Department of Electrical and Computer Engineering
|
| Lab |
Computational Bioinformatics & Bio-imaging Laboratory
|
| Street address |
4300 Wilson Blvd., Suite 750
|
| City |
Arlington |
| State/province |
VA |
| ZIP/Postal code |
22203 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE20700 |
Multi-level Support Vector Regression analysis to identify condition-specific regulatory networks |
|
