|
Status |
Public on Mar 10, 2010 |
Title |
MCF-7 P-62 |
Sample type |
RNA |
|
|
Source name |
Epithelial cells derived from metastatic adenocarcinoma
|
Organism |
Homo sapiens |
Characteristics |
gender: Female age: 69 years race: Caucasian cell line: MCF-7
|
Treatment protocol |
Stripped cells were grown over 96 hours (4 successive days) in phenol-red free IMEM media supplemented with 5% Charcoal-Stripped Calf Serum. Every 24 hours the media was removed and replaced with fresh phenol-red free IMEM media supplemented with 5% Charcoal-Stripped Calf Serum (3 times total).
|
Growth protocol |
Cells were grown in complete IMEM media supplemented with 5% Fetal Bovine Serum. Both Control and Treated cells were kept in a 37C incubator with 5% C02 and regulated humidity.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Standard one-cycle Affymetrix protocol
|
|
|
Hybridization protocol |
Standard Affymetrix protocol
|
Scan protocol |
Standard Protocol for Affymetrix GeneChip Scanner 3000
|
Description |
n/a
|
Data processing |
PLIER
|
|
|
Submission date |
Mar 09, 2010 |
Last update date |
Mar 09, 2010 |
Contact name |
Li Lily Chen |
E-mail(s) |
lchen06@vt.edu
|
Phone |
703-387-6060
|
Fax |
703-528-5543
|
Organization name |
Virginia Tech
|
Department |
The Bradley Department of Electrical and Computer Engineering
|
Lab |
Computational Bioinformatics & Bio-imaging Laboratory
|
Street address |
4300 Wilson Blvd., Suite 750
|
City |
Arlington |
State/province |
VA |
ZIP/Postal code |
22203 |
Country |
USA |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE20700 |
Multi-level Support Vector Regression analysis to identify condition-specific regulatory networks |
|