|
| Status |
Public on Jul 14, 2022 |
| Title |
Control_actin_ rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Fly heads
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: Fly heads
genotype: Actin-gal4/+
|
| Growth protocol |
All flies were raised at 25°C in 12-hour light: 12-hour dark cycles on standard diets
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The flies were raised for three more days to recover from CO2 exposure and collected using dry ice. RNA was prepared using TRI Reagent (SIGMA) according to the manufacturer’s protocol
3' RNA-seq library was performed based on Guttman’s lab "RNAtag Seq protocol" (Shishkin, A. A. et al. Simultaneous generation of many RNA-seq libraries in a single reaction. Nat. Methods 12, 323–325 (2015).)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 550 |
| |
|
| Data processing |
Sequencing was done using Next seq 500 high output kit V2 75 cycles (illumina) on illumina’s NextSeq 500 system
RNA-seq reads were aligned by STAR to the full genome sequences of Drosophila Melanogaster (UCSC version dm3)
Reads were counted per annotated gene using ESET (End Sequencing analysis Toolkit)
Genome_build: dm3
Supplementary_files_format_and_content: Table with raw gene counts for every gene and every sample
|
| |
|
| Submission date |
Mar 23, 2021 |
| Last update date |
Jul 14, 2022 |
| Contact name |
Neta Herman |
| E-mail(s) |
neta.herman@mail.huji.ac.il, netah@migal.org.il
|
| Organization name |
Hebrew University Of Jerusalem, Migal
|
| Department |
Gentics, Nutrition
|
| Lab |
Shagiv Shifman; Opatovsky Itai
|
| Street address |
Kiryat Shemona
|
| City |
Jerusalem, Kiryat Shemona |
| ZIP/Postal code |
1101202 |
| Country |
Israel |
| |
|
| Platform ID |
GPL22106 |
| Series (2) |
| GSE169412 |
The chromatin factor ROW cooperates with BEAF-32 in regulating long-range genes [RNA_seq_flyheads] |
| GSE169415 |
The chromatin factor ROW cooperates with BEAF-32 in regulating long-range genes |
|
| Relations |
| BioSample |
SAMN18437794 |
| SRA |
SRX10417985 |
