|
| Status |
Public on Feb 29, 2012 |
| Title |
wild type fruit, plant 2, biol rep2 (control5_IMG) |
| Sample type |
RNA |
| |
|
| Source name |
35th DAP immature green fruit of wild type plant
|
| Organism |
Solanum lycopersicum |
| Characteristics |
variety: Pusa Ruby
genotype/variation: wild type
age: 35 days after planting (DAP)
developmental stage: immature green fruit
tissue: pericarp
|
| Treatment protocol |
Untreated seedlings were used for shoot tissue
|
| Growth protocol |
Seedlings were raised on MS medium without antibiotic selection for 15 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 450C on GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Gene Chip Scanner 3000
|
| Description |
Gene expression data of wild type fruit, plant 2, biological rep. 2
|
| Data processing |
First-order analysis was performed using Affymetrix Gene Chip Operating (GCOS) software 1.2.1. Normalization of data and differential expression analysis was done using Avadis 4.2 prophetic software (Strandgenomics, Bangalore, India)
|
| |
|
| Submission date |
Mar 10, 2010 |
| Last update date |
Feb 29, 2012 |
| Contact name |
arun k. sharma |
| Organization name |
Delhi university
|
| Department |
Plant Molecular Biology
|
| Lab |
AKS
|
| Street address |
Benito-juarez road
|
| City |
Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110021 |
| Country |
India |
| |
|
| Platform ID |
GPL4741 |
| Series (1) |
| GSE20720 |
Understanding the complexity of fruit ripening by transcriptome analysis of rin mutant fruit |
|
