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Sample GSM520241 Query DataSets for GSM520241
Status Public on Feb 29, 2012
Title rin mutant fruit, plant 2, biol rep2 (mutant8_IMG)
Sample type RNA
 
Source name 35th DAP immature green fruit of rin mutant plant
Organism Solanum lycopersicum
Characteristics variety: Pusa Ruby
genotype/variation: rin mutant
age: 35 days after planting (DAP)
developmental stage: immature green fruit
tissue: pericarp
Treatment protocol Untreated seedlings were used for shoot tissue
Growth protocol Seedlings were raised on MS medium without antibiotic selection for 15 days.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 450C on GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using the Affymetrix Gene Chip Scanner 3000
Description Gene expression data of rin mutant fruit, plant 2, biological rep. 2
Data processing First-order analysis was performed using Affymetrix Gene Chip Operating (GCOS) software 1.2.1. Normalization of data and differential expression analysis was done using Avadis 4.2 prophetic software (Strandgenomics, Bangalore, India)
 
Submission date Mar 10, 2010
Last update date Feb 29, 2012
Contact name arun k. sharma
Organization name Delhi university
Department Plant Molecular Biology
Lab AKS
Street address Benito-juarez road
City Delhi
State/province Delhi
ZIP/Postal code 110021
Country India
 
Platform ID GPL4741
Series (1)
GSE20720 Understanding the complexity of fruit ripening by transcriptome analysis of rin mutant fruit

Data table header descriptions
ID_REF
VALUE GCRMA normalised signal intensity

Data table
ID_REF VALUE
RPTR-Les-A00196-1_s_at 3.5923703
RPTR-Les-A06498-1_at 3.5717847
RPTR-Les-A12338-1_at 3.0768483
RPTR-Les-AB076373-1_at 3.9573572
RPTR-Les-AE009032-1_at 3.1325393
RPTR-Les-AF062997-1_at 3.7828825
RPTR-Les-AF292559-1_at 3.7462585
RPTR-Les-AF292559-2_s_at 7.456623
RPTR-Les-AF292559-3_s_at 5.576368
RPTR-Les-AF292559-4_s_at 3.7365003
RPTR-Les-AF292560-1_s_at 5.8129373
RPTR-Les-AF298789-1_at 3.794215
RPTR-Les-AF323980-1_at 4.3641644
RPTR-Les-AF403737-1_at 4.7181077
RPTR-Les-AJ002682-1_s_at 3.6278183
RPTR-Les-AJ002682-2_s_at 3.514188
RPTR-Les-AJ132968-1_at 3.7029207
RPTR-Les-AY056050-1_at 3.6730502
RPTR-Les-AY125353_at 3.5710452
RPTR-Les-AY342347_at 3.764918

Total number of rows: 10209

Table truncated, full table size 285 Kbytes.




Supplementary file Size Download File type/resource
GSM520241.CEL.gz 850.9 Kb (ftp)(http) CEL
Processed data included within Sample table

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