|
| Status |
Public on Jul 01, 2021 |
| Title |
AML#3 MSCs |
| Sample type |
RNA |
| |
|
| Source name |
Mesenchymal stromal cells isolated from BM of pediatric patients at diagnosis
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Mesenchymal stromal cells (MSCs)
disease state: acute myeloid leukemia (AML)
|
| Treatment protocol |
No treatment
|
| Growth protocol |
MSCs were cultured in StemMACS™ MSC Expansion Media Kit XF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol starting from 100ng of Total RNA using the Affymetrix 3' IVT Plus Kit
|
| |
|
| Hybridization protocol |
15 ug were fragmented; following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Scan GCS3000.
|
| Description |
Gene expression data from MSCs isolated from BM at diagnosis of leukemia
|
| Data processing |
The data were normalized using R software [www.r-ptoject.org] with BioConductor package [www.bioconductor.org] using RMA
|
| |
|
| Submission date |
Mar 23, 2021 |
| Last update date |
Jul 01, 2021 |
| Contact name |
silvia bresolin |
| E-mail(s) |
silvia.bresolin@unipd.it
|
| Phone |
+390498215487
|
| Organization name |
università di padova
|
| Department |
department of women's and children's health
|
| Lab |
SSD ematologia-clinica sperimentale
|
| Street address |
via giustiniani 3
|
| City |
padova |
| State/province |
padova |
| ZIP/Postal code |
35128 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE169428 |
Targeting mesenchymal stromal cells plasticity to reroute acute myeloid leukemia course |
|
