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Sample GSM5202984 Query DataSets for GSM5202984
Status Public on Jul 01, 2021
Title AML#6 MSCs
Sample type RNA
 
Source name Mesenchymal stromal cells isolated from BM of pediatric patients at diagnosis
Organism Homo sapiens
Characteristics cell type: Mesenchymal stromal cells (MSCs)
disease state: acute myeloid leukemia (AML)
Treatment protocol No treatment
Growth protocol MSCs were cultured in StemMACS™ MSC Expansion Media Kit XF
Extracted molecule total RNA
Extraction protocol RNA was extracted using Trizol
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol starting from 100ng of Total RNA using the Affymetrix 3' IVT Plus Kit
 
Hybridization protocol 15 ug were fragmented; following fragmentation, 11 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Scan GCS3000.
Description Gene expression data from MSCs isolated from BM at diagnosis of leukemia
Data processing The data were normalized using R software [www.r-ptoject.org] with BioConductor package [www.bioconductor.org] using RMA
 
Submission date Mar 23, 2021
Last update date Jul 01, 2021
Contact name silvia bresolin
E-mail(s) silvia.bresolin@unipd.it
Phone +390498215487
Organization name università di padova
Department department of women's and children's health
Lab SSD ematologia-clinica sperimentale
Street address via giustiniani 3
City padova
State/province padova
ZIP/Postal code 35128
Country Italy
 
Platform ID GPL570
Series (1)
GSE169428 Targeting mesenchymal stromal cells plasticity to reroute acute myeloid leukemia course

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1007_s_at 8.278187043
1053_at 7.378817246
117_at 5.252883171
121_at 7.646342255
1255_g_at 4.619862835
1294_at 7.934324033
1316_at 5.977442155
1320_at 5.645423614
1405_i_at 6.034623098
1431_at 5.006040768
1438_at 7.075474516
1487_at 7.766720935
1494_f_at 5.594691852
1552256_a_at 7.888081662
1552257_a_at 8.095423652
1552258_at 5.508885382
1552261_at 4.179531326
1552263_at 6.926579217
1552264_a_at 8.34131381
1552266_at 3.274769254

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM5202984_MSC33_HG-U133_Plus_2.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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