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| Status |
Public on Mar 26, 2021 |
| Title |
13_F3_S221 |
| Sample type |
SRA |
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| Source name |
Blood, Infection only
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| Organism |
Capra hircus |
| Characteristics |
tissue type: Whole blood
treatment: infection only
Sex: male
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| Extracted molecule |
total RNA |
| Extraction protocol |
Blood samples were collected from blood of five goats in four treatment groups (No infection, Infection, Infection ZA inject, and Infection AB inject), following seven (7) dpi. Blood samples included red blood cells, white blood cells (total and differential), hemoglobin, platelets, and plasma protein, from the jugular vein in EDTA tubes.Total RNA was collected from blood samples using a modified TRIzol reagent procedure.
The cDNA libraries were constructed by initial first strand synthesis using the Protocol for Non-directional RNA-seq Workflows and NEBNEXT® UltraTM II RNA First Strand Synthesis Module (E7771), according to a modified manufacturer’s protocol.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
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| Description |
Blood cell-transcripts
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| Data processing |
Several methods were utilized to conduct bioinformatic analysis of the obtained sequence data. For gene expression analyses, we used the Partek® Flow® software suites pipelines that include, but are not limited to the STAR algorithm, Normalization, and the gene set differential analysis method (GSA).
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| Submission date |
Mar 25, 2021 |
| Last update date |
Mar 26, 2021 |
| Contact name |
Yonathan Tilahun |
| E-mail(s) |
yonathan.tilahun@langston.edu
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| Phone |
4054666105
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| Organization name |
Langston University
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| Department |
Extension
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| Lab |
Teaching Laboratory
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| Street address |
100 Success Dr.
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| City |
Langston |
| State/province |
OK |
| ZIP/Postal code |
73050 |
| Country |
USA |
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| Platform ID |
GPL21299 |
| Series (1) |
| GSE169607 |
Functional genome and microbiome in blood of goats affected by the gastrointestinal pathogen Haemonchus contortus |
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| Relations |
| BioSample |
SAMN18489574 |
| SRA |
SRX10442219 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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