|
| Status |
Public on Jan 11, 2022 |
| Title |
WT_media_2_1 |
| Sample type |
SRA |
| |
|
| Source name |
BMDC
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: BMDC
genotype: wild type
treatment: medium
|
| Treatment protocol |
Five biological replicates with treatments for 8 hours.
|
| Growth protocol |
Bone marrow derive dendritic cells were routinely generated by re-plating d9 bone marrow cell differentiation cultures (typically 2–3 × 106 cells into 100mm diameter dish) on uncoated plastic in complete RPMI1640 (Gibco) supplemented with 20 ng/ml of GM-CSF (PeproTech).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
BMDCs were harvest, live CD11c hi BMDCs were sorted into lysis buffer, flash frozen on dry ice. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Data processing |
Bcl2Fastq
RSEM Gene quantification V1.2.8
Genome_build: mm10
|
| |
|
| Submission date |
Mar 25, 2021 |
| Last update date |
Jan 11, 2022 |
| Contact name |
Ayshwarya Subramanian |
| E-mail(s) |
subraman@broadinstitute.org
|
| Organization name |
Broad Institute
|
| Department |
Klarman Cell Observatory
|
| Street address |
415 Main Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL9250 |
| Series (2) |
| GSE169663 |
Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Bat3-/- BMDC Transcriptomes III |
| GSE169664 |
Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Bat3-/- BMDC Transcriptomes |
|
| Relations |
| BioSample |
SAMN18492527 |
| SRA |
SRX10447034 |
