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Sample GSM5216914 Query DataSets for GSM5216914
Status Public on Apr 17, 2021
Title Joint_healthy_untreated_rep10
Sample type RNA
 
Source name Healthy
Organism Mus musculus
Characteristics disease state: Healthy
tissue: Knee joint
age: 3 months
gender: Male
strain: C57BL/6
treatment: untreated
Treatment protocol N/A
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Equal mass of (1) synovial tissue and (2) articular cartilage and subchondral bone were stored in RNAlater Stabilization Solution (AM7020, Invitrogen) at -80°C until further processing. Samples were homogenized with the Tissuelyser 2 from Qiagen (Redwood City, CA) before total RNA from the was extracted using Qiagen RNeasy Kit for purification of total RNA according to the manufacturer's instructions. mRNA expression analysis with the NanoString nCounter® Mouse Inflammation v2 Panel was performed according to the manufacturer's protocol (NanoString Technologies Inc., Seattle, WA).
Label N/A
Label protocol N/A
 
Hybridization protocol standard NanoString protocol
Scan protocol standard NanoString protocol
Data processing The raw Nanostring nCounter genes were normalized using nSolver 4.0 (NanoString Technologies Inc., Seattle, WA), adjusting for positive controls, housekeeping genes and background level.
 
Submission date Mar 29, 2021
Last update date Apr 19, 2021
Contact name Sean K Bedingfield
Organization name Vanderbilt University
Department Biomedical Engineering
Street address 2301 Vanderbilt Place PMB 351826
City Nashville
State/province TN
ZIP/Postal code 37235-1826
Country USA
 
Platform ID GPL27566
Series (1)
GSE171031 Matrix-anchored Nanoparticles for MMP13 RNA Interference Block Post-Traumatic Osteoarthritis

Data table header descriptions
ID_REF
VALUE RNA counts

Data table
ID_REF VALUE
Ager 66
Alox12 479
Alox15 695
Alox5 604
Areg 9
Arg1 59
Atf2 1215
Bcl2l1 1245
Bcl6 247
Birc2 454
C1qa 1874
C1qb 3815
C1ra 103
C1s 875
C2 171
C3 4605
C3ar1 972
C4a 1755
C6 48
C7 242

Total number of rows: 268

Table truncated, full table size 2 Kbytes.




Supplementary file Size Download File type/resource
GSM5216914_20180711_1801-SB-Cart1_10_10.RCC.gz 3.2 Kb (ftp)(http) RCC
Processed data included within Sample table

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