|
| Status |
Public on Apr 17, 2021 |
| Title |
Joint_PTOA_negative-ctrl-siRNA-treated_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Negative Ctrl siRNA
|
| Organism |
Mus musculus |
| Characteristics |
disease state: post-traumatic osteoarthritis (PTOA)
tissue: Knee joint
age: 3 months
gender: Male
strain: C57BL/6
treatment: negative-ctrl-siRNA
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Equal mass of (1) synovial tissue and (2) articular cartilage and subchondral bone were stored in RNAlater Stabilization Solution (AM7020, Invitrogen) at -80°C until further processing. Samples were homogenized with the Tissuelyser 2 from Qiagen (Redwood City, CA) before total RNA from the was extracted using Qiagen RNeasy Kit for purification of total RNA according to the manufacturer's instructions. mRNA expression analysis with the NanoString nCounter® Mouse Inflammation v2 Panel was performed according to the manufacturer's protocol (NanoString Technologies Inc., Seattle, WA).
|
| Label |
N/A
|
| Label protocol |
N/A
|
| |
|
| Hybridization protocol |
standard NanoString protocol
|
| Scan protocol |
standard NanoString protocol
|
| Data processing |
The raw Nanostring nCounter genes were normalized using nSolver 4.0 (NanoString Technologies Inc., Seattle, WA), adjusting for positive controls, housekeeping genes and background level.
|
| |
|
| Submission date |
Mar 29, 2021 |
| Last update date |
Apr 19, 2021 |
| Contact name |
Sean K Bedingfield |
| Organization name |
Vanderbilt University
|
| Department |
Biomedical Engineering
|
| Street address |
2301 Vanderbilt Place PMB 351826
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37235-1826 |
| Country |
USA |
| |
|
| Platform ID |
GPL27566 |
| Series (1) |
| GSE171031 |
Matrix-anchored Nanoparticles for MMP13 RNA Interference Block Post-Traumatic Osteoarthritis |
|
