|
| Status |
Public on Mar 02, 2011 |
| Title |
WholeCell_HeatShock_45min_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Whole cells
|
| Organism |
Chlamydomonas reinhardtii |
| Characteristics |
strain: CC-124
|
| Treatment protocol |
For heat shock, a 20 ml culture with ~4 × 106 cells per ml in a 100 ml Erlenmeyer flask was incubated at 42°C for 45 min in the light with shaking.
|
| Growth protocol |
Strains were grown heterotrophically in Tris-Acetate-Phosphate (TAP) medium (Harris, 1989) on a rotary shaker at 23°C either in the dark or under continuous irradiation with white light (fluence rate 45 μEm-2s-1) provided by fluorescent tubes (Osram L36W/25).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
As described in von Gromoff et al., 1989, Mol Cell Biol 9: 3911-3918
|
| Label |
Cy3
|
| Label protocol |
0.5 µg of total RNA were reverse transcribed into cDNA, and Cy3 labeled using the Quick Amp Labeling Kit, one-color (Agilent 5190-0442)
|
| |
|
| Hybridization protocol |
Hybridization was done with 0.6 µg labeled cDNA per array according to the Agilent protocol for 8x15k single color microarrays at a temperature of 65˚C overnight.
|
| Scan protocol |
An Agilent G2565CA DNA microarray scanner was used to scan arrays at 5 μm resolution, Feature Extraction Software 9.5.3.1 was then used to process and analyse array images.
|
| Description |
n/a
|
| Data processing |
The raw data was analyzed using GeneSpring GX 11.0 (Normalization: Shift to 75.0 percentile; Baseline Transformation: Median of all samples).
|
| |
|
| Submission date |
Mar 12, 2010 |
| Last update date |
Mar 02, 2011 |
| Contact name |
Bjoern Voss |
| Organization name |
University of Freiburg
|
| Department |
Institute of Biology III
|
| Lab |
Genetics
|
| Street address |
Schänzlestr. 1
|
| City |
Freiburg |
| ZIP/Postal code |
79104 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10173 |
| Series (2) |
| GSE20859 |
Change in gene expression in Chlamydomonas reinhardtii upon heat shock |
| GSE20861 |
Change in gene expression in Chlamydomonas reinhardtii upon heat shock and feeding with hemin and Mg-protoporphyrin |
|
