|
| Status |
Public on Mar 31, 2022 |
| Title |
BH0153-1 (WT) |
| Sample type |
RNA |
| |
|
| Source name |
Fetal livers
|
| Organism |
Mus musculus |
| Characteristics |
age: E14.5
tissue: liver
genotype: WT
|
| Treatment protocol |
EKLF knock-out gene to compare with WT cells
|
| Growth protocol |
Primary fetal liver cells without culture
|
| Extracted molecule |
total RNA |
| Extraction protocol |
E14.5 mouse fetal livers from WT and Eklf-/- mouse fetuses were homogenized by repeated pipetting in phosphate-buffered saline (PBS) (10 mM phosphate, 0.15 M NaCl [pH 7.4]). Total RNAs were then isolated with Trizol reagent (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
standard protocols
|
| |
|
| Hybridization protocol |
biotinylated cRNA was fragmented and hybridized to Mouse Genome 430 2.0 Array following the standard protocols
|
| Scan protocol |
Genome-scale gene expression profiling using the Mouse Genome Array 430A 2.0 (Affymetrix, Inc.).
|
| Description |
Gene expression data from embryos fetal liver
|
| Data processing |
Standard MAS5.0 method was applied to normalize the gene expression data. Gene expression values were log-transformed for later comparative analysis. Statistical analysis was carried out using R 3.0.2 language (R Development Core Team, 2013, http://www.R-project.org)
|
| |
|
| Submission date |
Mar 30, 2021 |
| Last update date |
Mar 31, 2022 |
| Contact name |
Chun-Hao James Hung |
| E-mail(s) |
libur777@gate.sinica.edu.tw
|
| Phone |
0930304978
|
| Organization name |
Institute of Molecular Biology, Academia Sinica
|
| Lab |
C.-K. James Shen
|
| Street address |
128 Academia Road, Section 2, Nankang, Taipei, Taiwan
|
| City |
Taipei |
| State/province |
TAIWAN |
| ZIP/Postal code |
11529 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE171200 |
Microarray expression level comparison between WT and KO fetal liver cells |
| GSE171289 |
A Positive Regulatory Feedback Loop Between EKLF/ KLF1 and TAL1/SCL Sustaining the Erythropoiesis |
|
