|
| Status |
Public on Mar 18, 2010 |
| Title |
The abomasal mucosa of calf#966 reinfected with Ostertagia ostertagi for 14 days |
| Sample type |
RNA |
| |
|
| Source name |
Drug-attenuated 5th reinfection
|
| Organism |
Bos taurus |
| Characteristics |
breed: Holstein
gender: bull calf
age: 8-9 months
|
| Treatment protocol |
drug-attenuated 5th reinfection
|
| Growth protocol |
Indoor fed with standard ration
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol followed with DNase digestion and Qiagen RNeasy purification
|
| Label |
Cy3
|
| Label protocol |
Cy3-labeled nonamer priming
|
| |
|
| Hybridization protocol |
single dye
|
| Scan protocol |
Axon GenePix 4000B at 5 uM resolution
|
| Description |
see Li et al., 2006
|
| Data processing |
The data presented here are normalized fluorescent intensities from either 4 or 8 spots of perfect match oligos. The raw data are log-transformed and then subject to quantitle normalization using R package
|
| |
|
| Submission date |
Mar 16, 2010 |
| Last update date |
Mar 17, 2010 |
| Contact name |
Robert W Li |
| E-mail(s) |
robert.li@ars.usda.gov
|
| Phone |
301-504-5185
|
| Fax |
301-504-8414
|
| Organization name |
USDA
|
| Department |
Animal & Natural Resources Institute
|
| Lab |
Bovine Functional Genomics Laboratory
|
| Street address |
Barc East
|
| City |
Beltsville |
| State/province |
MD |
| ZIP/Postal code |
20705 |
| Country |
USA |
| |
|
| Platform ID |
GPL3301 |
| Series (1) |
| GSE20927 |
Localized complement activation in the development of protective immunity against Ostertagia ostertagi infections in cattle |
|
