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Sample GSM523965 Query DataSets for GSM523965
Status Public on Aug 17, 2010
Title Mouse lung-embryo day 16-rep2
Sample type RNA
 
Source name embryo lung
Organism Mus musculus
Characteristics strain: ICR
gender: female
development stage: embryo day 16
Treatment protocol Lungs were isolated by manual dissection with the aid of a dissecting microscope where necessary. Lungs were washed twice with PBS before total RNA extraction
Growth protocol ICR female mice were bred for timed pregnancies. The day of plug observation was considered day 0 of gestation.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Labeling was performed according to Affymetrix Gene Chip technical manual
 
Hybridization protocol The targets for Affymetrix DNA microarray analysis were prepared according to the manufacturer’s instructions. Biotin-labeled cRNA, produced by in vitro transcription, was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays at 45°C for 16 hr and then washed and stained using the GeneChip Fluidics.
Scan protocol The arrays were scanned by a GeneArray Scanner and patterns of hybridization detected as light emitted from the fluorescent reporter groups incorporated into the target and hybridized to oligonucleotide probes.
Description Gene expression data from different lung development stages
Data processing The data were analyzed with Partek Genomics Suite 6.4 using Affymetrix default analysis settings and GC-RMA as normalization method
 
Submission date Mar 18, 2010
Last update date Aug 17, 2010
Contact name Jie Dong
E-mail(s) dong.jie@mayo.edu
Organization name Mayo Clinic
Department Thoracic Surgery
Street address 200 1st SW
City Rochester
State/province MN
ZIP/Postal code 55901
Country USA
 
Platform ID GPL1261
Series (2)
GSE20954 mRNA expression profile in mouse lung development
GSE21053 mRNA and microRNA expression profile in mouse lung development

Data table header descriptions
ID_REF
VALUE GCRMA normalized signal intensity

Data table
ID_REF VALUE
1415670_at 10.48
1415671_at 10.11
1415672_at 11.5885
1415673_at 8.71937
1415674_a_at 9.58849
1415675_at 9.48382
1415676_a_at 11.4141
1415677_at 9.65908
1415678_at 10.4052
1415679_at 11.4099
1415680_at 10.0348
1415681_at 10.0573
1415682_at 8.61633
1415683_at 11.0554
1415684_at 8.71068
1415685_at 9.47225
1415686_at 9.69649
1415687_a_at 10.7658
1415688_at 10.6124
1415689_s_at 10.4004

Total number of rows: 45101

Table truncated, full table size 850 Kbytes.




Supplementary file Size Download File type/resource
GSM523965.cel.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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