|
| Status |
Public on Aug 17, 2010 |
| Title |
Mouse lung-embryo day 18-rep2 |
| Sample type |
RNA |
| |
|
| Source name |
embryo lung
|
| Organism |
Mus musculus |
| Characteristics |
strain: ICR
gender: female
development stage: embryo day 18
|
| Treatment protocol |
Lungs were isolated by manual dissection with the aid of a dissecting microscope where necessary. Lungs were washed twice with PBS before total RNA extraction
|
| Growth protocol |
ICR female mice were bred for timed pregnancies. The day of plug observation was considered day 0 of gestation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Labeling was performed according to Affymetrix Gene Chip technical manual
|
| |
|
| Hybridization protocol |
The targets for Affymetrix DNA microarray analysis were prepared according to the manufacturer’s instructions. Biotin-labeled cRNA, produced by in vitro transcription, was fragmented and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays at 45°C for 16 hr and then washed and stained using the GeneChip Fluidics.
|
| Scan protocol |
The arrays were scanned by a GeneArray Scanner and patterns of hybridization detected as light emitted from the fluorescent reporter groups incorporated into the target and hybridized to oligonucleotide probes.
|
| Description |
Gene expression data from different lung development stages
|
| Data processing |
The data were analyzed with Partek Genomics Suite 6.4 using Affymetrix default analysis settings and GC-RMA as normalization method
|
| |
|
| Submission date |
Mar 18, 2010 |
| Last update date |
Aug 17, 2010 |
| Contact name |
Jie Dong |
| E-mail(s) |
dong.jie@mayo.edu
|
| Organization name |
Mayo Clinic
|
| Department |
Thoracic Surgery
|
| Street address |
200 1st SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55901 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE20954 |
mRNA expression profile in mouse lung development |
| GSE21053 |
mRNA and microRNA expression profile in mouse lung development |
|
