|
| Status |
Public on Mar 18, 2011 |
| Title |
E14L-16 |
| Sample type |
RNA |
| |
|
| Source name |
layer skeletal muscle collected at embyonic day 14
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: skeletal muscle
breed: layer
age: embyonic day 14
|
| Treatment protocol |
skeletal muscles (pectoralis) were collected from broilers and layers at E10, E12, E14 and E18 stages, immediately frozen in liquid nitrogen, and stored at -80°C until RNA isolation.
|
| Growth protocol |
Meat-type broiler eggs (commercial Arbor Acres) and egg-type layer eggs (White Leghorn) were incubated at 37.5°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA were isolated from skeletal muscles (pectoralis) collected from broilers and layers at the embryonic stages E10, E12, E14 and E18 with TRiZol reagent.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
|
| |
|
| Hybridization protocol |
Affymetrix Gene Chip array hybridization was carried out according to Affymetrix Expression Analysis Technical Manual by GeneTech Biotechnology Limited Company (Shanghai, China). Following labeled cRNA fragmentation, 15 μg of cRNA were hybridized for 16 hr at 45℃ on Affymetrix Chicken Genome Array. Washing and staining were followed standard Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
|
| Description |
gene expression data from layer skeletal muscle collected at embyonic day 14
|
| Data processing |
RMA with CDF file annotated by Dai et al.
|
| |
|
| Submission date |
Mar 22, 2010 |
| Last update date |
Mar 18, 2011 |
| Contact name |
Tingting Li |
| E-mail(s) |
litt@hsc.pku.edu.cn
|
| Phone |
+86-10-82801585
|
| Organization name |
Peking University
|
| Department |
Medical informatics
|
| Street address |
38 Xueyuan Rd
|
| City |
Beijing |
| ZIP/Postal code |
100191 |
| Country |
China |
| |
|
| Platform ID |
GPL10234 |
| Series (2) |
| GSE20990 |
Systematic identification of genes involved in embyonic chicken muscle development |
| GSE20992 |
Systematic analysis of miRNA transcriptome of skeletal muscle identifies novel miRNAs and differentially expressed miRNAs in divergent skeletal muscle growth rates of broiler and layer chickens |
|
