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Sample GSM525489 Query DataSets for GSM525489
Status Public on Dec 31, 2011
Title (764)Morphology:Femoral artery of pig#9006
Sample type RNA
 
Source name Femoral artery of pig#9006
Organism Sus scrofa
Characteristics artery: Femoral artery
pig id: 9006
cell type: blood, whole
age: 2 year
Growth protocol Pigs were on a normal diet
Extracted molecule total RNA
Extraction protocol RNA was isolated using Macherey Nagel Nucleo Spin II for the piglets and CsCl ultracentrifugation followed by MN Nucleospin II for the one and two year olds.
Label Biotin
Label protocol Biotinylated cRNA were prepared by the GeneChip One-Cycle target labeling kit according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
 
Hybridization protocol Following fragmentation, 6.5 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Porcine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 (affymetrix).
Description Femoral artery of pig#9006
Data processing Default GCOS 1.4 raw probe intensities were analyzed using the mas5 module of the affy 1.0 package for R version 2.3.1. The probesets that were present according to MAS5calls and normalized, background corrected, and summarized using the RMA function in Bionconductor.
 
Submission date Mar 24, 2010
Last update date Dec 31, 2011
Contact name Martin Bahls
E-mail(s) mbahls@purdue.edu
Organization name Purdue University
Street address 800 W Stadium Ave
City West Lafayette
ZIP/Postal code 47906
Country USA
 
Platform ID GPL3533
Series (1)
GSE21043 Gene expression differences between the brachial and femoral artery of the Rapacz HC swine

Data table header descriptions
ID_REF
VALUE normalized data as described in the data processing

Data table
ID_REF VALUE
AFFX-BioB-3_at 10.10727835
AFFX-BioB-5_at 10.16593272
AFFX-BioB-M_at 10.43783951
AFFX-BioC-3_at 10.4817465
AFFX-BioC-5_at 10.60816186
AFFX-BioDn-3_at 13.09643681
AFFX-BioDn-5_at 11.31270742
AFFX-CreX-3_at 13.99608866
AFFX-CreX-5_at 13.74312508
AFFX-DapX-3_at 9.638063856
AFFX-DapX-5_at 8.243938788
AFFX-DapX-M_at 9.666886415
AFFX-LysX-3_at 7.665278635
AFFX-LysX-5_at 5.204660043
AFFX-LysX-M_at 5.788780014
AFFX-PheX-3_at 7.383203191
AFFX-PheX-5_at 6.162049568
AFFX-PheX-M_at 6.598915258
AFFX-r2-Bs-dap-3_at 9.695364326
AFFX-r2-Bs-dap-5_at 8.409269956

Total number of rows: 19459

Table truncated, full table size 563 Kbytes.




Supplementary file Size Download File type/resource
GSM525489.CEL.gz 2.2 Mb (ftp)(http) CEL
GSM525489.CHP.gz 127.9 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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