NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM526122 Query DataSets for GSM526122
Status Public on Aug 17, 2010
Title E14-1cardA/B
Sample type RNA
 
Source name embryo lung
Organism Mus musculus
Characteristics strain: ICR
gender: female
development stage: embryo day 14
Treatment protocol Lungs were isolated by manual dissection with the aid of a dissecting microscope where necessary. Lungs were washed twice with PBS before total RNA extraction
Growth protocol ICR female mice were bred for timed pregnancies. The day of plug observation was considered day 0 of gestation.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using mir VanaTM miRNA Isolation Kit (Ambion) according to the manufacturer's instructions.
Label SYBR
Label protocol none
 
Hybridization protocol not applicable
Scan protocol Raw miRNA array data was analyzed by using RQ manager software on the ABI system
Description micro expression data from different lung development stages
reverse_transcription_protocol: MiRNA was reverse transcibed to cDNA using the Megaplex TM RT Rodent Primers Pool and the TaqMan MicroRNA Reverse Transcription Kit
real-time_PCR: Quantitative 384 well TaqMan Low Density Array real-time PCR was run on the ABI PRISM 7900 System using the TaqMan Universal PCR Master Mix
Data processing Normalized CT (ΔCT) was calculated by comparing each miRNA value to that of small nuclear U6 RNA. The U6 RNA is a common internal control for each microRNA array card.
 
Submission date Mar 24, 2010
Last update date Aug 17, 2010
Contact name Jie Dong
E-mail(s) dong.jie@mayo.edu
Organization name Mayo Clinic
Department Thoracic Surgery
Street address 200 1st SW
City Rochester
State/province MN
ZIP/Postal code 55901
Country USA
 
Platform ID GPL10253
Series (2)
GSE21052 microRNA expression profile in mouse lung development
GSE21053 mRNA and microRNA expression profile in mouse lung development

Data table header descriptions
ID_REF
VALUE Normalized CT (ΔCT)

Data table
ID_REF VALUE
396 3.2380962
2308 4.2131405
2228 4.2203274
2535 5.5282173
2459 5.1970615
1644 5.0878067
391 5.2265396
402 5.21294
1821 6.2236633
395 6.2106304
2278 6.2442245
580 6.234186
1319 7.124466
1629 6.02359
430 7.2177067
2300 7.2288227
2304 7.2391624
419 6.202753
2299 7.2367954
2260 8.218954

Total number of rows: 516

Table truncated, full table size 5 Kbytes.




Supplementary file Size Download File type/resource
GSM526122_E14-1cardA.sds.txt.gz 7.5 Kb (ftp)(http) TXT
GSM526122_E14-1cardB.sds.txt.gz 5.7 Kb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap