|
| Status |
Public on Aug 17, 2010 |
| Title |
E14-2cardA/B |
| Sample type |
RNA |
| |
|
| Source name |
embryo lung
|
| Organism |
Mus musculus |
| Characteristics |
strain: ICR
gender: female
development stage: embryo day 14
|
| Treatment protocol |
Lungs were isolated by manual dissection with the aid of a dissecting microscope where necessary. Lungs were washed twice with PBS before total RNA extraction
|
| Growth protocol |
ICR female mice were bred for timed pregnancies. The day of plug observation was considered day 0 of gestation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using mir VanaTM miRNA Isolation Kit (Ambion) according to the manufacturer's instructions.
|
| Label |
SYBR
|
| Label protocol |
none
|
| |
|
| Hybridization protocol |
not applicable
|
| Scan protocol |
Raw miRNA array data was analyzed by using RQ manager software on the ABI system
|
| Description |
micro expression data from different lung development stages
reverse_transcription_protocol: MiRNA was reverse transcibed to cDNA using the Megaplex TM RT Rodent Primers Pool and the TaqMan MicroRNA Reverse Transcription Kit
real-time_PCR: Quantitative 384 well TaqMan Low Density Array real-time PCR was run on the ABI PRISM 7900 System using the TaqMan Universal PCR Master Mix
|
| Data processing |
Normalized CT (ΔCT) was calculated by comparing each miRNA value to that of small nuclear U6 RNA. The U6 RNA is a common internal control for each microRNA array card.
|
| |
|
| Submission date |
Mar 24, 2010 |
| Last update date |
Aug 17, 2010 |
| Contact name |
Jie Dong |
| E-mail(s) |
dong.jie@mayo.edu
|
| Organization name |
Mayo Clinic
|
| Department |
Thoracic Surgery
|
| Street address |
200 1st SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55901 |
| Country |
USA |
| |
|
| Platform ID |
GPL10253 |
| Series (2) |
| GSE21052 |
microRNA expression profile in mouse lung development |
| GSE21053 |
mRNA and microRNA expression profile in mouse lung development |
|
