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Sample GSM5293152 Query DataSets for GSM5293152
Status Public on May 11, 2024
Title Day_3_FSHD2_4_R2 [methylation]
Sample type SRA
 
Source name Primary myoblasts from quadricep biopsy
Organism Homo sapiens
Characteristics cell line: FSHD2-4
cell type: Myoblasts
muscle group: quadricep
fshd status: FSHD2
day of differentiation: Day 3
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was collected from a single 6 cm dish using the DNeasy Blood & Tissue kit (69504, Qiagen).
All DNA methylation data was generated using the TruSeq Methyl Capture EPIC kit (FC-151-1003, Illumina) according to manufacturer’s protocol.
Libraries were sequenced on the Illumina NextSeq500 with paired-end 75 bp reads to a depth of 20 to 82 million reads.
 
Library strategy Bisulfite-Seq
Library source genomic
Library selection RANDOM
Instrument model Illumina NextSeq 500
 
Description PerMeth.AllRegions.csv
Data processing Raw data converted to fastq using bcl2fastq v2.18.0.12 from Illumina.
Raw reads from TruSeq Methyl Capture EPIC libraries were mapped to canonical chromosomes from hg38 and the patch region of D4Z4 (chr4_KQ983257v1_fix) using bismark (version 0.19.0).
Sites were extracted from the bam file using bismark_methylation_extractor with paired end and no overlap specified. To remove bias from the ends of reads, 2 bp from the 5’ end of read 2 and 1 bp from the 3’ end of read 1 were ignored when extracting sites.
All CpG sites were read into methylKit (version 1.16.0). Sites within 200 bp were merged into one region using bumphunter (version 1.32.0). Methylation over those regions was summarized using regionCounts and filtered for at least three CpG sites.
Regions were then filtered for a coverage of at least 5. Regions were normalized using normalizeCoverage, and only regions with coverage in all samples were kept.
Genome_build: hg38 (GRCh38)
Supplementary_files_format_and_content: *.bismark.cov: Coverage files are output from bismark and contain the 0-indexed site, percent methylation, number of methylated sites and number of unmethylated sites in that order.
Supplementary_files_format_and_content: PerMeth.AllRegions.csv: Percent methylation file contains the percent methylation for regions per sample normalized as described above.
 
Submission date May 12, 2021
Last update date May 11, 2024
Contact name Ali Mortazavi
E-mail(s) ali.mortazavi@uci.edu
Organization name UC Irvine
Street address 2300E Biological Sciences 3
City Irvine
State/province CA
ZIP/Postal code 92697
Country USA
 
Platform ID GPL18573
Series (2)
GSE174369 Muscle group specific transcriptomic and DNA methylation differences related to developmental patterning influence FSHD [methylation]
GSE174370 Muscle group specific transcriptomic and DNA methylation differences related to developmental patterning influence FSHD
Relations
BioSample SAMN19132179
SRA SRX10863193

Supplementary file Size Download File type/resource
GSM5293152_Day_3_FSHD2_4_R2_R1_bismark_bt2_pe.bismark.cov.gz 53.3 Mb (ftp)(http) COV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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