|
| Status |
Public on Dec 31, 2011 |
| Title |
total microRNAs in LNCaP cells treated with DHT at 4h |
| Sample type |
RNA |
| |
|
| Source name |
LNCaP cell line
|
| Organism |
Homo sapiens |
| Characteristics |
treatment group: dihydrotestosterone (DHT) at 4h
hormone status: androgen-sensitive
tissue: prostate cancer
cell line: LNCaP
|
| Treatment protocol |
Before androgen treatment, the LNCaP cells were cultivated with Phenol Red -free RPMI 1640 (GIBCO/ BRL) supplemented with 10% charcoal-dextran-stripped FBS for 5 days. Then the cells were induced with androgen dihydrotestosterone (DHT), at ultimate concentration 10 nM, which has been proven by pre-experiment to be optimum for LNCaP cell growth (data not shown). The cell response to androgen treatment was analyzed at ten time points — 0h, 20min, 40min, 1h, 2 h, 4 h, 8 h, 16h, 24 h and 48 h, where ‘0h’ refers to cell status before androgen action.
|
| Growth protocol |
The androgen-sensitive human prostate cancer cell line LNCaP was obtained from ATCC and maintained in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and penicillin (100 units/ml)-streptomycin (100g/ml) at 37 ℃ in a humidified 5% CO2 cell incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For cell samples at each time point, the total RNA was extracted and purified using the RNeasy Mini kit following the manufacturer’s instructions. The isolated RNA was stored at -70℃ for the Illumina microarrays.
|
| Label |
biotin
|
| Label protocol |
The isolated RNA was stored at -70℃ for the Illumina microarrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Apr 07, 2010 |
| Last update date |
Dec 31, 2011 |
| Contact name |
Wenjuan Mo |
| E-mail(s) |
041019012@fudan.edu.cn
|
| Phone |
021-65100243
|
| Fax |
021-65100243
|
| Organization name |
Fudan University
|
| Department |
Life School
|
| Lab |
Genetics national lab
|
| Street address |
No. 220 Handan Road
|
| City |
Shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
| |
|
| Platform ID |
GPL8933 |
| Series (1) |
| GSE21245 |
Genome-wide analysis of matched microRNA-mRNA time-course data after androgen injection to prostate cancer LNCaP cell line |
|
