|
Status |
Public on Feb 01, 2011 |
Title |
Hoxa9-ER on day 4, rep 1 |
Sample type |
RNA |
|
|
Source name |
Murine bone marrow transduced with epitope tagged forms of Hoxa9 on day 4
|
Organism |
Mus musculus |
Characteristics |
cell type: Hoxa9-ER cells gender: female age: 6-8 week old strain: C57BL/6 cell line: murine bone marrow transduced with epitope tagged forms of Hoxa9 time point: day 4
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Standard Affymetrix protocol.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner.
|
Description |
Gene expression data from murine cell line transduced with epitope tagged forms of Hoxa9 day 4
|
Data processing |
The data were analyzed with robust multi-array average (RMA) normalization using default background adjustment and quantile normalization settings.
|
|
|
Submission date |
Apr 12, 2010 |
Last update date |
Sep 13, 2011 |
Contact name |
Jay Hess |
E-mail(s) |
jayhess@med.umich.edu
|
Phone |
734-763-6384
|
Fax |
734-763-4782
|
URL |
http://www.pathology.med.umich.edu/faculty/Hess/index.html
|
Organization name |
University of Michigan
|
Department |
Pathology
|
Street address |
1301 Catherine
|
City |
Ann Arbor |
State/province |
MI |
ZIP/Postal code |
48109 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (2) |
GSE21299 |
Expression data from murine cell line transduced with epitope tagged forms of Hoxa9 |
GSE33518 |
Identification and characterization of Hoxa9 binding sites in hematopoietic cells |
|