|
| Status |
Public on Oct 18, 2010 |
| Title |
Myeloma-20 |
| Sample type |
genomic |
| |
|
| Source name |
CD138+ bone marrow cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: bone marrow
cell type: Multiple myeloma plasma cell
myeloma translocation: t(11;14)
|
| Treatment protocol |
all cells were derived from patients prior to treatment with chemotherapy
|
| Growth protocol |
cell lines were grown in RPMI media with 10% FCS
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted using the AllPrep protocol (Qiagen)
|
| Label |
DNP/biotin
|
| Label protocol |
as per manufacturers (Illumina) instructions
|
| |
|
| Hybridization protocol |
as per manufacturers (Illumina) instructions
|
| Scan protocol |
as per manufacturers (Illumina) instructions
|
| Description |
bisulfite modified genomic DNA
|
| Data processing |
files were analyzed in GenomeStudio (Illumina) as per manufacturers (Illumina) instructions using the methylation module v1.5.5, and are described as beta values
|
| |
|
| Submission date |
Apr 12, 2010 |
| Last update date |
Oct 18, 2010 |
| Contact name |
Brian A Walker |
| E-mail(s) |
brian.walker@icr.ac.uk
|
| Organization name |
The Institute of Cancer Research
|
| Department |
Haemato-Oncology Research Unit
|
| Lab |
Molecular Haematology Team
|
| Street address |
15 Cotswold Road
|
| City |
Sutton |
| ZIP/Postal code |
SM2 5NG |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL8490 |
| Series (1) |
| GSE21304 |
Aberrant global methylation patterns affect the molecular pathogenesis and prognosis of human multiple myeloma |
|
