|
| Status |
Public on Jul 07, 2010 |
| Title |
C1S/84 - repeat 4 - rev. label - mAdbID:107314 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
WT 84 Control - cy5
|
| Organism |
Nakaseomyces glabratus |
| Characteristics |
strain: 84
developmental stage: log-phase culture
genotype/variation: Wild Type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol Extraction Protocol
Other: Total RNA was isolated from log phase culture of C. glabrata grown in YPD using Trizole (Invitrogen) and RNeasy MiniElute cleanup kit (Qiagen).
|
| Label |
cy5
|
| Label protocol |
Cy5 Labeling Protocol
Other: Ten micrograms of total RNA were used for each labeling. The RNA was reversed transcribed to cDNA to incorporate the fluorescent Cy5-dUTP (GE Health Care).
|
| |
|
| Channel 2 |
| Source name |
Complemented strain C1S- cy3
|
| Organism |
Nakaseomyces glabratus |
| Characteristics |
strain: C1S
developmental stage: log-phase culture
genotype/variation: CgPDR1 complemented
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol Extraction Protocol
Other: Total RNA was isolated from log phase culture of C. glabrata grown in YPD using Trizole (Invitrogen) and RNeasy MiniElute cleanup kit (Qiagen).
|
| Label |
cy3
|
| Label protocol |
Cy3 Labeling Protocol
Other: Ten micrograms of total RNA were used for each labeling. The RNA was reversed transcribed to cDNA to incorporate the fluorescent Cy3-dUTP (GE Health Care).
|
| |
|
| |
| Hybridization protocol |
Agilent Hybridization
Other: According to the manufacture's recommended protocol (Two-Color Microarray-Based Gene Expression Analysis, Agilent).
|
| Scan protocol |
Agilent Scanning Protocol
Other: Arrays were scanned at 5um resolution and 100/10% PMT XDR on an Agilent DNA Microarray Scanner G2505B.
|
| Description |
mAdb experiment ID: 107314
|
| Data processing |
Agilent Data Processing Protocol
Calculation Method: Images were auto gridded, analyzed and data extracted using Agilent Feature Extraction Software. Spot values were normalized using the default linear-lowess normalization.
|
| |
|
| Submission date |
Apr 15, 2010 |
| Last update date |
Jul 07, 2010 |
| Contact name |
Huei-Fung Tsai |
| E-mail(s) |
HT28T@NIH.GOV
|
| Phone |
301-496-8993
|
| Fax |
301-480-0512
|
| Organization name |
NIH/NIAID
|
| Department |
LCID
|
| Lab |
CMS
|
| Street address |
10 Center Dr. Bldg. 10 Rm. 11N228
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL10325 |
| Series (1) |
| GSE21355 |
Azole Resistance Mechanism in Candida glabrata |
|
