|
| Status |
Public on Mar 25, 2011 |
| Title |
ILD_14 |
| Sample type |
RNA |
| |
|
| Source name |
Lung tissue sample from the patient with ILD
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: NSIP
fvc-group: FVC3
tissue: lung
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from individual human lung samples by homogenization in TRIzol® Reagent per the manufacturer’s protocol (Invitrogen, Carlsbad, CA) using Soft Tissue Omni Homogenizer Tips (Omni International; Marietta, GA).
|
| Label |
biotin
|
| Label protocol |
Total RNA (100 ng) was used to synthesize double-stranded cDNA using the Two-Cycle cDNA Synthesis Kit (Affymetrix, Santa Clara, CA). The cDNA served as a template to generate biotin-labeled antisense cRNA with an IVT Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Labeled cRNA was fragmented and hybridized to the Affymetrix GeneChip® Human Genome U133 Plus 2.0 Array as described in the Affymetrix GeneChip® protocol.
|
| Scan protocol |
The chips were scanned using an Affymetrix GeneChip® Scanner 3000 7G, and each digitized image was processed using the GeneChip® Operating Software (GCOS) version 1.2 (Affymetrix).
|
| Description |
ILD patient
|
| Data processing |
Gene expression data was summarized, normalized, and transformed in log2-scale by the GCRMA method
|
| |
|
| Submission date |
Apr 16, 2010 |
| Last update date |
Mar 25, 2011 |
| Contact name |
Ji-Hoon Cho |
| E-mail(s) |
jcho@systemsbiology.org
|
| Organization name |
Institute for Systems Biology
|
| Street address |
401 Terry Avenue North
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE21369 |
Gene expression profiles of interstitial lung disease (ILD) patients |
| GSE21411 |
Systems biology of interstitial lung diseases |
|
