|
| Status |
Public on May 23, 2024 |
| Title |
Bladder tissue biopsy from IC/BPS patient 2 |
| Sample type |
SRA |
| |
|
| Source name |
Bladder tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Bladder
disease state: Interstitial cystitis/bladder pain syndrome with Hunner lesions
group: HIC
|
| Treatment protocol |
No treatment was performed
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Single-cell suspensions were converted to barcoded scRNA-seq libraries by using the Chromium Single Cell 3’ Library, Gel Bead & Multiplex Kit, and Chip Kit (10X Genomics), aiming for an estimated 10,000 cells per library. Samples were processed using kits pertaining to V3 barcoding chemistry of 10X Genomics.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
BJB2
|
| Data processing |
Basecalls performed using CASAVA
Data analysis was performed with CellRanger 3.0
Genome_build: Hg38
Supplementary_files_format_and_content: mtx files were generated from CellRanger
Supplementary_files_format_and_content: tsv files were generated from CellRanger
|
| |
|
| Submission date |
May 25, 2021 |
| Last update date |
May 23, 2024 |
| Contact name |
Fei Su |
| Organization name |
Shanghai Jiao Tong University
|
| Department |
School of Life Science and Biotechnology
|
| Street address |
800 Dongchuan Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200240 |
| Country |
China |
| |
|
| Platform ID |
GPL20795 |
| Series (1) |
| GSE175526 |
Single cell RNA-seq of bladder tissue |
|
| Relations |
| BioSample |
SAMN19332826 |
